Frozen Cell Crusher: Cryo-Press
TOP > Products > Frozen Cell Crusher
Actual Performance of Cryo-Press in DNA and RNA Extractions.
DNA extraction by Cryo-Press
 |
 |
| Microscopic image of Nannochloris Bacillaris (before homogenizing) | Microscopic image of Nannochloris Bacillaris (after homogenizing) |
The size of nannochlorisbacillaris is about 5 microns. (DAPI staining, 345 nm exposure, magnification: 1,000x)
DNA (blue color) appeared after homogenizing. Conventionally, it took 2 to 3 hours to homogenize the sample with a mortar. However, by Cryo-Press, it takes only 2 minutes (15 seconds x 8 times) to homogenize the sample.
In the image of "after homogenizing", blue fluorescence show DNA. The DNA are isolated from the cells by successful homogenization.
Experimented by:
Laboratory of Plant Life System, Department of Integrated Biosciences Graduate School of Frontier Sciences, The University of Tokyo
RNA extraction by Cryo-Press
Conventional Homogenizing has a high risk of the degradation of RNAs by the heat of homogenizing, however, this image shows that CRYO-PRESS extracts rRNAs of 28S and 18S, which shows clear proportion (2:1).
Experimented by:
Tissue Engineering Laboratory, The University of Tokyo
The difference between Cryo-Press and Glass Homogenizer
We homogenized chicken sciatic nerve in two ways (Cryo-Press and conventional glass homogenizer), and extracted the proteins after adding SDS.
1. Comparison of the amount of the extracts
| Homogenizing method | Sciatic nerve | Skeletal muscle |
|---|---|---|
| Cryo-Press | 4.44 mg/ml | 9.67 mg/ml |
| Glass homogenizer | 2.94 mg/ml | 5.38 mg/ml |
2. The patterns of gel electrophoresis
 |
Sciatic nerve a. Glass homogenizer b. Cryo-Press |
| Skeletal muscle c. Glass homogenizer d. Cryo-Press |
|
| On each lane, we ran the proteins which come from the tissue of 0.23mg. | |
Both of these data show that CYRO-PRESS works better than conventional glass homogenizer. And also, Cryo-Press is good for time-saving. We hit the samples only once or twice with the power of air compressor.
