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G. HEINEMANN
Ultraschall-
und Labortechnik
Erwin-Rommel-Str. 42
73525 Schwaebisch Gmuend
Germany
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DNA
extraction by CRYO-PRESS
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Microscopic image of
Nannochloris Bacillaris
(before homogenizing)
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Microscopic image of
Nannochloris Bacillaris
(after homogenizing) |
The size
of nannochlorisbacillaris is about 5 microns.
(DAPI staining, 345 nm exposure, magnification: 1,000x)
DNA(blue color) appeared after
homogenizing. Conventionally, it took 2 to 3 hours
to homogenize the sample with a mortar. However, by Cryo-Press,
it takes only 2 minutes
(15 seconds x 8 times) to homogenize the sample.
In the image of "after homogenizing", blue fluorescence
show DNA. The DNA are isolated from the cells by successful
homogenization.
Experimented by:
Laboratory of Plant Life System,
Department of Integrated Biosciences Graduate School of Frontier
Sciences,
The University of Tokyo
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RNA
extraction by CRYO-PRESS
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Conventional Homogenizing has
a high risk of the degradation of RNAs by the heat of homogenizing,
however, this image shows that CRYO-PRESS extracts rRNAs of
28S and 18S, which shows clear proportion (2:1).
Experimented by:
Tissue Engineering
Laboratory,
The University of Tokyo
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See the difference
between CRYO-PRESS and Glass Homogenizer.
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We homogenized chicken sciatic nerve in two ways
(CRYO-PRESS and conventional Glass Homogenizer), and
extracted the proteins after adding SDS.
1. Comparison of the amount of the extracts
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homogenizing method
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sciatic nerve
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skeletal muscle
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CRYO-PRESS
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4.44mg/ml
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9.67mg/ml
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Glass Homogenizer
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2.94mg/ml
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5.38mg/ml
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2. The patterns of gel electrophoresis
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sciatic nerve
a. Glass homogenizer
b. CRYO-PRESS
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skeletal muscle
c. Glass Homogenizer
d. CRYO-PRESS |
| On each lane, we ran
the proteins which come from the tissue of 0.23mg. |
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Both of these data show that CYRO-PRESS works better than
conventional Glass Homogenizer. And also, CRYO-PRESS is good
for timesaving. We hit the samples only once or twice with
the power of air compressor.
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